› 1ifs

X-ray diffraction
2Å resolution

RICIN A-CHAIN (RECOMBINANT) COMPLEX WITH ADENOSINE (ADENOSINE BECOMES ADENINE IN THE COMPLEX)

Released:
DOI: 10.2210/pdb1ifs/pdb
PDB entry 1ifs coloured by chain, front view.
PDB entry 1ifs coloured by chain, side view.
PDB entry 1ifs coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
X-ray structure of recombinant ricin A-chain at 1.8 A resolution.
Weston SA, Tucker AD, Thatcher DR, Derbyshire DJ, Pauptit RA
J Mol Biol 244 410-22 (1994)
PMID: 7990130

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.46 KDa
Source organism: Ricinus communis
Expression system: Not provided
UniProt:
  • Canonical: P02879 (Residues: 36-298; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 68.7Å b: 68.7Å c: 141.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 not available
Expression system: Not provided

Quick links

Citations

14 review citations

The history of ricin, abrin and related toxins.
Olsnes S. (2004)
13 more

9 mentions without citation

Structures and Ribosomal Interaction of Ribosome-Inactivating Proteins.
Shi et al. (2016)
8 more