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{
    "metadata": {
        "accession": "P9WEI7",
        "id": "CLAR_AMPCV",
        "source_organism": {
            "taxId": "56467",
            "scientificName": "Ampulloclitocybe clavipes",
            "fullName": "Ampulloclitocybe clavipes (Club foot)"
        },
        "name": "Cytochrome P450 monooxygenase claR",
        "description": [
            "Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of clavilactone A, a meroterpenoid that features a unique benzo-fused ten-membered carbocyclic ring unit with an alpha,beta-epoxy-gamma-lactone moiety, forming an intriguing 10/5/3 tricyclic nested skeleton (PubMed:38602511). ClaR, ClaS and ClaT are sufficient to produce clavilactone A (PubMed:38602511). ClaR acts as a macrocyclase to catalyze the oxidative cyclization of the isopentenyl to the nonterpenoid moieties to form the benzo-fused macrocycle, leading to wigantol (PubMed:38602511). The biosynthesis begins with the prenyltransferase claS that transfers geranyl pyrophosphate (GPP) to hydroquinone to produces geranylhydroquinone. The cytochrome P450 monooxygenase claR then catalyzes the diradical coupling reaction between the intramolecular hydroquinone and allyl moieties to form the benzo-fused ten-membered carbocyclic ring unit of wigantol. Finally the cytochrome P450 monooxygenase claT exquisitely and stereoselectively assembles the alpha,beta-epoxy-gamma-lactone moiety, producing clavilactone A via arnebinol A (PubMed:38602511)"
        ],
        "length": 537,
        "sequence": "MDKHLSPGFASYHAQRILATPNVVTITEVALGIITLYLLGSYIVRRNSLPPGPRGLPIVGNVRDLPTERPWLFWAKHKDIYGPISSITVMGQSFIIVNELQIAIDLLDKKSAIYSDRPRLEFAGEMIGWNRQMILSQYGERFRTMRKFVKEFIGTKAAVVQYRPLQEIETRYFLARVLATPEHLADHLRLTAGAIFLRMSHGYAIDTEKPDALVNLVETAAKEFYIATSPGAWLVDQFPALQKLPNWFPGTHFKKVAAEFFEHNMEQADRPHEFVKRRMNAGTALPSFTSRMLERGLDEKDDEVVRWAANSLYGGGTDTVVASLSAFFLTMVLYPEVQKKAQKEIDTVIGTDRLPTLDDRSRLPYIEAVLKEVLRWHPIGPMGIPHRVTEDDVYNGYLIPKGAIVLPNLWMFAHDPSQYHNADEFKPERYLETDGNVPELDPHALAFGFGRRACPGQELADTNMFLTIAMSLAVFNISKAVDEKGREIEPVNEFSSGTVSHPKPYKCKVTPRSASAEELIRLVGDDHMNRPTDANDL",
        "proteome": null,
        "gene": "claR",
        "go_terms": [
            {
                "identifier": "GO:0004497",
                "name": "monooxygenase activity",
                "category": {
                    "code": "F",
                    "name": "molecular_function"
                }
            },
            {
                "identifier": "GO:0005506",
                "name": "iron ion binding",
                "category": {
                    "code": "F",
                    "name": "molecular_function"
                }
            },
            {
                "identifier": "GO:0016705",
                "name": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen",
                "category": {
                    "code": "F",
                    "name": "molecular_function"
                }
            },
            {
                "identifier": "GO:0020037",
                "name": "heme binding",
                "category": {
                    "code": "F",
                    "name": "molecular_function"
                }
            }
        ],
        "protein_evidence": 1,
        "source_database": "reviewed",
        "is_fragment": false,
        "in_alphafold": true,
        "in_bfvd": false,
        "ida_accession": "a02e61ba6c64bb9a9c779746e03a6c43264a16fb",
        "counters": {
            "domain_architectures": 520850,
            "entries": 13,
            "isoforms": 0,
            "proteomes": 0,
            "sets": 1,
            "structures": 0,
            "taxa": 1,
            "dbEntries": {
                "cathgene3d": 1,
                "pfam": 1,
                "ssf": 1,
                "cdd": 1,
                "panther": 1,
                "prosite": 1,
                "prints": 2,
                "interpro": 5
            },
            "proteome": 0,
            "taxonomy": 1,
            "similar_proteins": 520850
        }
    }
}