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{
"metadata": {
"accession": "P9WEG1",
"id": "MFMB_ANNMO",
"source_organism": {
"taxId": "326622",
"scientificName": "Annulohypoxylon moriforme",
"fullName": "Annulohypoxylon moriforme (Filamentous fungus)"
},
"name": "Non-reducing polyketide synthase mfmB",
"description": [
"Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the phthalide-terpenoid hybrid 11'-O-desmethylfendlerol (PubMed:38404388). Within the pathway, mfmB catalyzes the first step that leads to the synthesis of 3,5-dimethylorsellinic acid (DMOA) via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (PubMed:38404388). DMOA is then transformed into the phthalide 5,7-dihydroxy-4-(hydroxymethyl)-6-methylphthalide by the cytochrome P450 monooxygenase mfmA and the hydrolase mfmC. Subsequently, the methyltransferase mfmE catalyzes 7-O-methylation to yield 5-hydroxy-4-(hydroxymethyl)-7-methoxy-6-methylphthalide, which undergoes C-3 hydroxylation by the cytochrome P450 monooxygenase mfmF. The resultant cyclopolic acid (2,5-dihydroxy-4-(hydroxymethyl)-7-methoxy-6-methylphthalide) is then farnesylated by the DMATS-type prenyltransferase mfmD to afford 5-O-farnesylcyclopolic acid. Finally, the Pyr4-family terpene cyclase mfmH cyclizes the farnesyl moiety of 5-O-farnesylcyclopolic acid into a drimane-like structure, thus completing the biosynthesis of 11'-O-desmethylfendlerol (PubMed:38404388)"
],
"length": 2493,
"sequence": "MTQFGTSPISRTQEFVVALFGPQVTHWTQDSLSNLQSDLLGNPNLEFVAKSLLELPSLWSELEQEYDFEGSRGAHTLKELHDFATGEKSLDPQTLTNTHLAPLTVVYQVIDFIRSAGNPGTKESLRGFEAAQGFCIGFLSAAALASANDWSDFERNVSNAIRLATCIGIIVDAEEASHPAQDRATAISVHWKTAADRTYLETCLDNVPEAYISCITDDRNLTVTLPKDHEEAFCARLREDKISTTSVGLNGCYHHSRHVEAANKLAKLCTANSDLRLPSAGSLRLPLRSTADGSIIKNGALHEIAIQLILCKRAHWFQTVKSTLGDLTPDKATFVPLGTKSCIPRSLSAIRYNHDDENALEEIAVVGMACRFPQADTLEEFWEIIQAGKTALSTMPLERFNPADITREPKLPEYWGNFLRRPDAFDHRFFGISGREAKSMDPQQRLALQVAYEALESSGYCVNETSSRVTDVGCYLGVGSVDYEDNIESENANAFAATGMLRAFISGRISHFYGWTGPSITFDTACSSSAVAIHTACKALLGGECSMALAGGVNVITSPTLHQNLAGASFLNPNGSSRAFDTMAGGYCRGEGAGILVLKPLSRAVADGDTVLGVIAASAVNQGSNCSPITVPDSWSQSSLYERVLTTARIKPTEVTYVEAHGTGTPVGDPIEYESVRMALTGPSRNSELFLGSVKDSIGHTEAASGVAAVIKTLLMMQHKTIPKQANFVSLNPRIKASSSNKIVVPKTTQPWTSQKHVALVNNYGAAGSNAAILVRAHSEVPLDSTSGVKTQDTSAVYPILLSAKSPSNLQSYVDALKLYLPQAKASLGGIAYNIARTHNSTFDNRIAFTASDIQSLSSGLNNFTISGGIITRTDKFPVVLCFGGQTGRKVTVSKDLYDNCDIFRKHLDECNAVCHQLGLPSIFPEIFQGKDIEDIVTLHCMLLSLQISSAKSWIDSGLEVDTVIGHSFGQLTALCIAGSISLEDTFRLISGRARIIRDSWNTESGAMLSVECDKEEIEAVAKRLNTTSDYRVDIACYNGPRSFVLAGDARSVDKAEEECSSFKKTRLQNTHAYHSYVADGILSDLKGLAESITLRPPRIRVETCSAGDNWSSFTADEVVKHTRQPVYFADAVERIATRLSSAIWLEAGSASPIVAMARRIIPKTDRSDVFIPIELGSDGATANLANAACQLWKAGSATQHWAFHKSSSSRYTRINLPPYQFDKTSHWIEYKPRTKVGVSENQNIVSKVSSLVSILRSKVKGENLFSVDTSNVIFQLAGQGHAVTGQSLCPASMYVELVVRCATLLLDAAYTAKAVPHVEGLTMSAPLGLGAGISVFIRLSNVAQGKWDFAIFSRSSINGDAESSETEHAKGIISCVPADDAVAEGRLKLLRRLARTSRIDRIKKLPSATGVSGAMVYKLFSDVVEYASYYRGVRNLSAVDNEAVGLVTVPTEQPLGMDAGICDPISLDNFLQVAGIHVNCLSPRKQDEVFMCTAVEEVIFSASFMANRGHSRSWIVYTRYETTSNSIIANDIFVCDAASKELVVAIMGATFRSVPFRSLSRSLSRLNGMPNTNIIAAVERSPQISDSDSDDADSEYQKGYTPLTEYEDEKPLTKHGYFSHTQSPLASLGIQQQPAEEPTESPDIVQKVRDMFSAIIEIPVEEIEPSSSLDDLGIDSLLVTEVLVEIQSRFGVKITQAQFQECTDVLDVARFIQPVEKTNKSQPPVEKTGVKHSFTEKPLNGASDPANRHGIDNINQIRENLAIVSRDSFIEAKPSYDQHAETTGFKNFNTEVFPLQSELVVQYVLAAFASLGCVVPDLKFGDEAPIIRFDPKHNKVVPQLYKILEDAGLLKKGEDGVLRRTEVPIQMVPTLPLHEKMLKLFPKHASETKLLYTTGHRLADCLSGAADPIALLFGDSDARALLADVYTNAPMFKTGTLLLSQYLTSVLKGFKGGRELKILELGAGTGGTTKDVVEKLAALGPEYKFSYTFTDLSSSLVAAARRKFAKWSFMRYTVVDIEKDPNPQFLGAYDIIISTNCIHATKNLVNSTTNIRKMLGPDGVLCLVELTRNLFWFDLVFGLLEGWWLFSDGRKHALASEGLWKRDLNAAGFNWVDWSDTSSSESDILRVITASPYAIGNKTADSLPIGDGLPLKETVTFKEVDGLELQADIYYPPKVVDSGRTLPVALMIHGGGHVMLSRNDIRPKQTNMLIKSGFLPISIDYRLCPEVTLPEGPMSDAADALLWIRTVLPKLTLSRHDVRVNAERVVAVGWSSGGHLAMSLAWTSQARQIQPPSAILAFYSPSDYEDSFWSEPNIPAGSRVMNGATSYELDDQVWAGISDHAIVGYNPPPTKRALGGWLAPTDPRSRLALYMNWHGRTLHTILGGLDKNDRKEPGTPAPELVKVISPLAQARAGNYATPTFLIHPREDDLVPWQQAERTWQALQATGTQAELRIVEDVPHLFDLYPEHQSNEVLTRVVAEGYEFLCQHVGLSD",
"proteome": null,
"gene": "mfmB",
"go_terms": [
{
"identifier": "GO:0016740",
"name": "transferase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0016746",
"name": "acyltransferase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0008236",
"name": "serine-type peptidase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0006508",
"name": "proteolysis",
"category": {
"code": "P",
"name": "biological_process"
}
},
{
"identifier": "GO:0004315",
"name": "3-oxoacyl-[acyl-carrier-protein] synthase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0006633",
"name": "fatty acid biosynthetic process",
"category": {
"code": "P",
"name": "biological_process"
}
}
],
"protein_evidence": 1,
"source_database": "reviewed",
"is_fragment": false,
"in_alphafold": true,
"in_bfvd": false,
"ida_accession": "246eda0d32f916cd6b19d5cbd363dec40021f198",
"counters": {
"domain_architectures": 7,
"entries": 58,
"isoforms": 0,
"proteomes": 0,
"sets": 9,
"structures": 0,
"taxa": 1,
"dbEntries": {
"cathgene3d": 7,
"profile": 3,
"pfam": 11,
"cdd": 2,
"ssf": 6,
"smart": 2,
"panther": 1,
"prosite": 2,
"interpro": 24
},
"proteome": 0,
"taxonomy": 1,
"similar_proteins": 7
}
}
}
