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InterPro-Version: 108.0
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{
"metadata": {
"accession": "A0A1V0QSE8",
"id": "ERIJ_HERER",
"source_organism": {
"taxId": "91752",
"scientificName": "Hericium erinaceus",
"fullName": "Hericium erinaceus (Lion's mane mushroom)"
},
"name": "UDP-glycosyltransferase eriJ",
"description": [
"UDP-glycosyltransferase; part of the gene cluster that mediates the biosynthesis of erinacines, cyathane-xylosides that show unique biological activities, including leishmanicidal activity, stimulating activity for nerve growth-factor synthesis, and agonistic activity toward the kappa opioid receptor (PubMed:28371074, PubMed:31535864). Within the pathway, eriJ tranfers xylose from UDP-xylose onto C-14 of 11-O-acetyl-cyathatriol to form eracine Q, and, at a lower rate, glucose from UDP-D-glucose to produce eracine Q2 (PubMed:31535864). The first step of the erinacines biosynthesis pathway is catalyzed by the geranylgeranyl diphosphate (GGPP) synthase eriE via conversion of farnesyl pyrophosphate and isopentyl pyrophosphate into geranylgeranyl pyrophosphate (GGPP). GGPP is then substrate of the diterpene cyclase eriG for the production of cyatha-3,12-diene. The cytochrome P450 monooxygenase eriI then hydroxylates cyatha-3,12-diene at C-14 of the seven-membered ring to produce erinacol, which is further hydroxylated at C-15 by the cytochrome P450 monooxygenase eriC to yield cyathadiol. The cytochrome P450 monooxygenase eriA then catalyzes C-11 hydroxylation in the presence of the short chain dehydrogenase/reductase (SDR) eriH, which leads to the production of cyathatriol. The acetyltransferase eriL converts cyathatriol into 11-O-acetyl-cyathatriol. The SDR eriH catalyzes further oxidation of 11-O-acetyl-cyathatriol into 1-O-acetylcyathin A3. Finally, the glycosyl transferase eriJ tranfers xylose from UDP-xylose onto C-14 of 11-O-acetyl-cyathatriol to form eracine Q. EriJ is also able to convert 11-O-acetyl-cyathatriol to eracine Q2 by using UDP-D-glucose as cosubstrate, but at a lower rate (Probable)"
],
"length": 506,
"sequence": "MSTQNYHIVAVPPNEWGHMRPMIAFLARLVAVSLNSVDITVTLIIAESAVAKARTELSIQLAGEGIQSAESRFEVVPTAVHMFWPADAYLPALKATYAEVIKKKEPDFALLESMIHPFFDVVRSSATKPIKVGAWLPVALPSWTSMAPICYIRDDPQAYVKQVETTMAEKGLGYMEAASDAYLSHVNGRVLRIPGFPEMTDYEGFPQEPPVLLPVAMVVDWVFGIRDADILVTSTAQALERQGLQVFSKWLKEQPKHSDILAVGPLTSQRTPEVARKEKEEADAGGFTAFLDAWAAKKGPKSVLYICFGSVLLPAEIEHLYAVMRVLLELQIPFIMVLSDAARAALPADLAAAVRDSGLVKLTPWAPQQYILAHAAVGWFLSHCGINGTLESLCLRVPMVCWPLFADQPVLSILVAQVYGCGYELGEVRKGFGLKYRASTGKTPGGTVEDVTREAREVFSKAFFNKAERAKVDANLEKMATELNAAWDAEGDARASALALLDFIRK",
"proteome": null,
"gene": "eriJ",
"go_terms": [
{
"identifier": "GO:0008194",
"name": "UDP-glycosyltransferase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
}
],
"protein_evidence": 1,
"source_database": "reviewed",
"is_fragment": false,
"in_alphafold": true,
"in_bfvd": false,
"ida_accession": "781f331c11f55b381926aa2b09b4b07d5b4d0620",
"counters": {
"domain_architectures": 97521,
"entries": 6,
"isoforms": 0,
"proteomes": 0,
"sets": 2,
"structures": 0,
"taxa": 1,
"dbEntries": {
"cathgene3d": 1,
"ssf": 1,
"cdd": 1,
"panther": 1,
"pfam": 1,
"interpro": 1
},
"proteome": 0,
"taxonomy": 1,
"similar_proteins": 97521
}
}
}
