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{
"metadata": {
"accession": "cd11643",
"entry_id": null,
"type": "domain",
"go_terms": null,
"source_database": "cdd",
"member_databases": null,
"integrated": "IPR012797",
"hierarchy": null,
"name": {
"name": "Precorrin-6A synthase (also named CobF)",
"short": "Precorrin-6A-synthase"
},
"description": [
{
"text": "<p>Precorrin-6A synthase participates in the pathway toward the biosynthesis of cobalamin (vitamin B12). There are two distinct cobalamin biosynthetic pathways in bacteria. The aerobic pathway requires oxygen, and cobalt is inserted late in the pathway; the anaerobic pathway does not require oxygen, and cobalt insertion is the first committed step towards cobalamin synthesis. This model represents CobF, the precorrin-6A synthase, an enzyme specific to the aerobic pathway. After precorrin-4 is methylated at C-11 by CobM to produce precorrin-5, CobF catalyzes the removal of the extruded acyl group in the subsequent step, and the addition of a methyl group at C-1. The product of this reaction is precorrin-6A, which gets reduced by an NADH-dependent reductase to yield precorrin-6B. This family includes enzymes in GC-rich Gram-positive bacteria, alpha proteobacteria and Pseudomonas-related species. [[cite:PUB00014672], [cite:PUB00000553], [cite:PUB00015657], [cite:PUB00079512], [cite:PUB00035309], [cite:PUB00009744], [cite:PUB00035310]]</p>",
"llm": false,
"checked": false,
"updated": false
}
],
"wikipedia": null,
"literature": {
"PUB00014672": {
"PMID": 11153269,
"ISBN": null,
"volume": "61",
"issue": null,
"year": 2001,
"title": "Multiple biosynthetic pathways for vitamin B12: variations on a central theme.",
"URL": null,
"raw_pages": "267-97",
"medline_journal": "Vitam Horm",
"ISO_journal": "Vitam. Horm.",
"authors": [
"Roessner CA",
"Santander PJ",
"Scott AI."
],
"DOI_URL": "http://dx.doi.org/10.1016/S0083-6729(01)61009-4"
},
"PUB00035309": {
"PMID": 16042605,
"ISBN": null,
"volume": "33",
"issue": "Pt 4",
"year": 2005,
"title": "Aerobic synthesis of vitamin B12: ring contraction and cobalt chelation.",
"URL": null,
"raw_pages": "815-9",
"medline_journal": "Biochem Soc Trans",
"ISO_journal": "Biochem. Soc. Trans.",
"authors": [
"Heldt D",
"Lawrence AD",
"Lindenmeyer M",
"Deery E",
"Heathcote P",
"Rigby SE",
"Warren MJ."
],
"DOI_URL": "http://www.biochemsoctrans.org/bst/033/bst0330815.htm"
},
"PUB00000553": {
"PMID": 9742225,
"ISBN": null,
"volume": "335 ( Pt 1)",
"issue": null,
"year": 1998,
"title": "Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon.",
"URL": null,
"raw_pages": "159-66",
"medline_journal": "Biochem J",
"ISO_journal": "Biochem. J.",
"authors": [
"Raux E",
"Lanois A",
"Warren MJ",
"Rambach A",
"Thermes C."
],
"DOI_URL": "http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=9742225&action=stream&blobtype=pdf"
},
"PUB00035310": {
"PMID": 12055304,
"ISBN": null,
"volume": "148",
"issue": "Pt 6",
"year": 2002,
"title": "Isolation and characterization of 14 additional genes specifying the anaerobic biosynthesis of cobalamin (vitamin B12) in Propionibacterium freudenreichii (P. shermanii).",
"URL": null,
"raw_pages": "1845-53",
"medline_journal": "Microbiology",
"ISO_journal": "Microbiology (Reading, Engl.)",
"authors": [
"Roessner CA",
"Huang KX",
"Warren MJ",
"Raux E",
"Scott AI."
],
"DOI_URL": "http://mic.sgmjournals.org/cgi/content/abstract/148/6/1845"
},
"PUB00079512": {
"PMID": 12195810,
"ISBN": null,
"volume": "19",
"issue": "4",
"year": 2002,
"title": "The biosynthesis of adenosylcobalamin (vitamin B12).",
"URL": null,
"raw_pages": "390-412",
"medline_journal": "Nat Prod Rep",
"ISO_journal": "Nat Prod Rep",
"authors": [
"Warren MJ",
"Raux E",
"Schubert HL",
"Escalante-Semerena JC."
],
"DOI_URL": "http://dx.doi.org/10.1039/b108967f"
},
"PUB00009744": {
"PMID": 11215515,
"ISBN": null,
"volume": "57",
"issue": "13-14",
"year": 2000,
"title": "Biosynthesis of cobalamin (vitamin B12): a bacterial conundrum.",
"URL": null,
"raw_pages": "1880-93",
"medline_journal": "Cell Mol Life Sci",
"ISO_journal": "Cell. Mol. Life Sci.",
"authors": [
"Raux E",
"Schubert HL",
"Warren MJ."
],
"DOI_URL": "http://dx.doi.org/10.1007/PL00000670"
},
"PUB00015657": {
"PMID": 12869542,
"ISBN": null,
"volume": "278",
"issue": "42",
"year": 2003,
"title": "Comparative genomics of the vitamin B12 metabolism and regulation in prokaryotes.",
"URL": null,
"raw_pages": "41148-59",
"medline_journal": "J Biol Chem",
"ISO_journal": "J. Biol. Chem.",
"authors": [
"Rodionov DA",
"Vitreschak AG",
"Mironov AA",
"Gelfand MS."
],
"DOI_URL": "http://dx.doi.org/10.1074/jbc.M305837200"
}
},
"set_info": {
"accession": "cl00304",
"name": "TP_methylase"
},
"overlaps_with": null,
"counters": {
"subfamilies": 0,
"domain_architectures": 0,
"interactions": 0,
"matches": 4063,
"pathways": 0,
"proteins": 4063,
"proteomes": 2966,
"sets": 1,
"structural_models": {
"alphafold": 2819,
"bfvd": 0
},
"structures": 2,
"taxa": 4105
},
"entry_annotations": {},
"cross_references": {},
"is_llm": false,
"is_reviewed_llm": false,
"is_updated_llm": false,
"representative_structure": {
"accession": "2npn",
"name": "Crystal structure of putative cobalamin synthesis related protein (CobF) from Corynebacterium diphtheriae"
}
}
}
