Cellular Phenotype Database homepage

Cellular Phenotype Database


Measurements for AMBN10000310

Screen:
B1_SyM_1 (Primary)
Study:
BZH_SH4-mCherry-GFP
Reagent:
AMBN10000310
Gene mapped:
PIK3R2
Screen scoring method:
In the case of SH4-HASPB-GFP, ∼97% of the negative controls did not return scores above a threshold of 2.5. In the case of SH4-YES1-Cherry, 98% of the negative controls were below a threshold of 3.5. Based on these data, scores of 2.5 for SH4-HASPB-GFP and 3.5 for YES1-mCherry were chosen as suitable thresholds for initial identification of potential phenotypes.
Replicas:
№ Phenotypes ch_c ch_g ch_g_s gfp_c gfp_g gfp_g_s
1
  • Intracellular retention of SH4(HASPB)-GFP
  • Intracellular retention of SH4(YES)-mCherry
4.4 3.8 -0.5 3.6 2 0.5
2
  • No phenotype assigned
3.1 -0.2 1.5 1.7 1 0.8
Parameters/Rules:
Intracellular retention of SH4(HASPB)-GFP: gfp_g >=2.5 || gfp_g_s >=2.5 || gfp_c >=2.5
Intracellular retention of SH4(YES)-mCherry: ch_g >=3.5 || ch_g_s >=3.5 || ch_c >=3.5