E-TABM-909 - miRNA profiling of human acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment
Released on 19 February 2010, last updated on 2 May 2014
The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34+CD38- fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34+CD38- cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34+CD38- fraction.
microRNA profiling by array, individual strain or line
Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment. Laura Pedranzini; Federica Mottadelli; Simona Ronzoni; Franca Rossella; Manuela Ferracin; Ivana Magnani; Gaia Roversi; Patrizia Colapietro; Massimo Negrini; Pier Giuseppe Pelicci; Lidia Larizza.