E-TABM-531 - Transcription profiling of mice mutant for Sox8 and Sox9 has early testicular sterility
Released on 3 January 2013, last updated on 28 April 2015
To study a possible redundant role of mouse Sox8 and Sox9 after testis induction we generated double mutants by AMH-Cre conditional inactivation of Sox9 in a Sox8-/- background. Double mutants arrest spermatogenesis in early pubescence. We performed expression profiling to explore causes. AMH-Cre/+;Sox9flox/flox;Sox8-/- is a conditional homozygous knockout of SOX9 in a sox8-/- background, using an AMH-CRE line where the CRE recombinase is expressed under the promoter region of the human AMH gene in Sertoli cells starting at E13.5. The specific cross was between males of the genotype +/+;Sox9flox/flox;Sox8-/- and females of the genotype AMH-Cre/+;Sox9flox/flox;Sox8+/-.
transcription profiling by array, co-expression, development or differentiation, disease state, genetic modification, in vivo
Genome-wide identification of Sox8-, and Sox9-dependent genes during early post-natal testis development in the mouse. F. Chalmel,A. Lardenois,I. Georg, F. Barrionuevo,P. Demougin, B. Jegou, G. Scherer† and M. Primig. ANDROLOGY (2012), Europe PMC 23315995