E-MTAB-8880 - Histone modification profiling (using the iChIP protocol) for two markers of regulatory activity (H3K4me3, H3K27ac) from a panel of genetically diverse crosses of Drosophila melanogaster during embryogenesis

Status
Last updated on 3 December 2020, released on 4 December 2020
Organism
Drosophila melanogaster
Samples (133)
Protocols (4)
Description
Profiling of histone modifications with the iChIP protocol (PMID25103404) using antibodies against two markers of regulatory activity: H3K27ac (abcam, ab4729) and H3K4me3 (Millipore, 07-473). All paternal fly lines were taken from the Drosophila Genetic Reference Panel crossed to a common mother (PMID31308546). Data were collected at three time points (2-4h, 6-8h, 10-12h at 25C) with two biological replicates per collection.
Experiment types
ChIP-seq, development or differentiation design, replicate design, strain or line design
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-8880.idf.txt
Sample and data relationshipE-MTAB-8880.sdrf.txt
Links