E-MTAB-8646 - Genome-wide CRISPR knockout screen in Huh7.5.1 human hepatocytes for the identification of host factors of hepatitis A virus

Status
Submitted on 21 May 2018, last updated on 9 January 2020, released on 19 January 2020
Organism
Homo sapiens
Samples (4)
Protocols (4)
Description
A knockout cell library in Huh7.5.1 cells was generated by introducing a genome-scale CRISPR library (GeCKOv2, Addgene #1000000049) and subjected to hepatitis A virus infection (HM175/18f) to isolate virus-resistant mutant cells. Genomic DNA was isolated from the original and virus-selected mutant cell populations and abundance of guideRNA encoding sequences were measured by sequencing on an Illumina NextSeq (High Output).
Experiment types
genotyping by high throughput sequencing, genetic modification design, genotyping design
Contact
Citation
A CRISPR screen identifies UFMylation and TRAMP-like complexes required for hepatitis A virus infection. Jessie Kulsuptrakul, Ruofan Wang, Nathan L. Meyers, Melanie Ott, Andreas S. Puschnik.
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-8646.idf.txt
Sample and data relationshipE-MTAB-8646.sdrf.txt
Links