E-MTAB-8646 - Genome-wide CRISPR knockout screen in Huh7.5.1 human hepatocytes for the identification of host factors of hepatitis A virus
Submitted on 21 May 2018, last updated on 9 January 2020, released on 19 January 2020
A knockout cell library in Huh7.5.1 cells was generated by introducing a genome-scale CRISPR library (GeCKOv2, Addgene #1000000049) and subjected to hepatitis A virus infection (HM175/18f) to isolate virus-resistant mutant cells. Genomic DNA was isolated from the original and virus-selected mutant cell populations and abundance of guideRNA encoding sequences were measured by sequencing on an Illumina NextSeq (High Output).
genotyping by high throughput sequencing, genetic modification design, genotyping design
A CRISPR screen identifies UFMylation and TRAMP-like complexes required for hepatitis A virus infection. Jessie Kulsuptrakul, Ruofan Wang, Nathan L. Meyers, Melanie Ott, Andreas S. Puschnik.