E-MTAB-8581 - A cell atlas of human thymic development defines T cell repertoire formation
Last updated on 21 February 2020, released on 21 February 2020
Homo sapiens, Mus musculus
We performed single-cell RNA-sequencing to create a cell census of the human thymus during development, early childhood and adult life. We sampled 15 embryonic and fetal thymi spanning thymic developmental stages between 7 post-conception weeks (PCW) to 17 PCW, and 9 postnatal thymi from paediatric and adult samples. We compared the cellular composition and T cell differentiation within human and mouse thymus using newly generated and repository mouse datasets. Finally, we investigated the bias in the recombination and selection of human versus mouse TCR repertoires.
RNA-seq of coding RNA from single cells