E-MTAB-8560 - Single-cell RNA-sequencing of mouse thymic epithelial cells across the first year of life

Status
Submitted on 27 November 2019, last updated on 14 September 2020, released on 14 September 2020
Organism
Mus musculus
Samples (11682)
Protocols (6)
Description
The thymus shapes the T cell receptor repertoire, and is one of the first organs to rapidly age, with gross changes in cellularity and architecture. To resolve the nature of these changes we used SMART-Seq2 on FACS purified thymic epithelial cells (TEC) across the first year of mouse life. We sorted 4 TEC populations in each of 5 mice at each age (1 week, 4 weeks, 16 weeks, 32 weeks and 52 weeks old).
Experiment types
RNA-seq of coding RNA from single cells, cell type comparison design, observational design
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-8560.idf.txt
Sample and data relationshipE-MTAB-8560.sdrf.txt
Links