E-MTAB-8560 - Single-cell RNA-sequencing of mouse thymic epithelial cells across the first year of life
Submitted on 27 November 2019, last updated on 14 September 2020, released on 14 September 2020
The thymus shapes the T cell receptor repertoire, and is one of the first organs to rapidly age, with gross changes in cellularity and architecture. To resolve the nature of these changes we used SMART-Seq2 on FACS purified thymic epithelial cells (TEC) across the first year of mouse life. We sorted 4 TEC populations in each of 5 mice at each age (1 week, 4 weeks, 16 weeks, 32 weeks and 52 weeks old).
RNA-seq of coding RNA from single cells, cell type comparison design, observational design