E-MTAB-794 - Transcriptional profiling by array in rat of age-dependent gene expression in sensorimotor cortex following spinal cord injury

Status
Released on 15 January 2012, last updated on 2 May 2014
Organism
Rattus norvegicus
Samples (72)
Array (1)
Protocols (7)
Description
In this study, we investigated the extent and nature of the difference between the dynamic cortical gene expression profiles of aged (22-month-old) and young (2-month-old) rats following thoracic corticospinal tract (CST) transection and whether the anti-scarring-treatment (AST)-induced regeneration program can be activated in aged animals. GeneChip analysis was performed on layers V/VI of the rat sensorimotor cortex at 1, 7 and 35 days post operation (dpo), which represented the acute, subacute and chronic stages of SCI, respectively. Anti-scarring treatment (AST) includes local injections of an iron chelator and cAMP immediately after SCI. For our purpose we used the Affymetrix GeneChip Rat Gene 1.0 ST Array (A-AFFY-160) which contains approximately 27,000 genes.
Experiment types
transcription profiling by array, compound treatment, development or differentiation, injury, time series
Contacts
Citations
Age-dependent modulation of cortical transcriptomes in spinal cord injury and repair. Jaerve A, Kruse F, Malik K, Hartung HP, Müller HW. :e49812 (2012), PMID:23236355
Shaping age-specific cortical transcriptomes during spinal cord injury repair. Anne Jaerve; Fabian Kruse; Katharina Malik; Hans-Peter Hartung; Hans Werner Mueller.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-794.idf.txt
Sample and data relationshipE-MTAB-794.sdrf.txt
Raw data (2)E-MTAB-794.raw.1.zip, E-MTAB-794.raw.2.zip
Processed data (1)E-MTAB-794.processed.1.zip
Array designA-AFFY-160.adf.txt
R ExpressionSetE-MTAB-794.eSet.r