E-MTAB-7919 - Single cell RNA-seq profiling of T cells isolated from MC38 mouse tumors

Status
Last updated on 2 May 2019, released on 12 May 2019
Organism
Mus musculus
Samples (4)
Protocols (5)
Description
We investigate the single-cell landscape of the inflammatory mouse tumor model MC38, a C57BL/6 tumor cell line derived from colon adenocarcinoma. MC38 (diluted in HBSS and matrigel) was inoculated in the right unilateral flank (in the border of positions B2 and B3) of C57BL/6 mice (ref Study 16-3384 AV). Tumors were taken one day after group-out (average 150-250 mm3 at day 0), approximately 14-19 days. Tissues were dissociated and flow sorted accordingly to obtain the following groups for 10x Chromium 5' Gene Expression Profiling. Our results indicate that the degree of clonal expansion is correlated with expression of T cell exhaustion markers, and that T cells with strong exhaustion phenotype also express high levels of activation markers, such as interferon gamma.
Experiment types
RNA-seq of coding RNA from single cells, cell type comparison design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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