E-MTAB-7747 - ChIP-seq for H3K27ac in the GR18 cell line treated for 1.5 h with 1 µM dexamethasone

Status
Submitted on 25 January 2017, last updated on 11 March 2019, released on 11 March 2019
Organism
Homo sapiens
Samples (1)
Protocols (8)
Description
We performed ChIP-seq in the GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone)treatment for 90 minutes. Cells were cross-linked with 1% formaldehyde for 3 minutes.
Experiment types
ChIP-seq, binding site identification design
Contact
Citation
Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements. Verena Thormann, Laura V. Glaser, Maika C. Rothkegel, Marina Borschiwer, Melissa Bothe, Alisa Fuchs, Sebastiaan H. Meijsing.
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-7747.idf.txt
Sample and data relationshipE-MTAB-7747.sdrf.txt
Processed data (1)E-MTAB-7747.processed.1.zip
Links