E-MTAB-7747 - ChIP-seq for H3K27ac in the GR18 cell line treated for 1.5 h with 1 µM dexamethasone
Submitted on 25 January 2017, last updated on 11 March 2019, released on 11 March 2019
We performed ChIP-seq in the GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone)treatment for 90 minutes. Cells were cross-linked with 1% formaldehyde for 3 minutes.
ChIP-seq, binding site identification design
Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements. Verena Thormann, Laura V. Glaser, Maika C. Rothkegel, Marina Borschiwer, Melissa Bothe, Alisa Fuchs, Sebastiaan H. Meijsing.