E-MTAB-7574 - Microarray on Caenorhabditis elegans N2 and CB4856 infected with Orsay virus
Released on 8 July 2020, last updated on 6 August 2020
This experiment investigates the gene expression differences upon Orsay virus infection in the Caenorhabdits elegans strains N2 and CB4856. Assays measuring viral load found that the N2 strain displays higher viral loads upon infection than the CB4856 strain. The goal of the experiment was to identify gene-expression differences that could explain the differences in viral load. We (mock-)infected 26h-old C. elegans populations with Orsay virus and took samples after 30h of infection. For each treatment-strain combination 8 samples were collected. Thereafter RNA was isolated, labelled, and hybridized on microarray.
transcription profiling by array, disease state design, strain or line design
Balancing selection shapes the Intracellular Pathogen Response in natural Caenorhabditis elegans populations. Lisa Van Sluijs, Kobus Bosman, Frederik Pankok, Tatiana Blokhina, Joost AG Riksen, Basten L Snoek, Gorben P Pijlman, Jan E Kammenga, Mark G Sterken.