E-MTAB-7524 - Bulk RNA Barcoding and sequencing (BRB-seq) protocol for RNA-seq of 60 human LCL from the 1000G project

Status
Released on 17 December 2018, last updated on 24 April 2019
Organism
Homo sapiens
Samples (61)
Protocols (7)
Description
Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bulk RNA-seq procedure. For this we use BRB-seq protocol on 60 human LCL data from the 1000G project samples, in order to show its multiplexing capacity and compare its effectiveness to already published TruSeq results on same samples.
Experiment types
RNA-seq of coding RNA, genotype design
Contact
Citation
BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. Alpern D, Gardeux V, Russeil J, Mangeat B, Meireles-Filho ACA, Breysse R, Hacker D, Deplancke B. 20(1):71 (2019)
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-7524.idf.txt
Sample and data relationshipE-MTAB-7524.sdrf.txt
Links