E-MTAB-7411 - 3prime RNA-seq of murine SCLC cell line modified with CRISPR-activation of Myc, Mycl or Mycn

Status
Last updated on 8 July 2019, released on 9 July 2019
Organism
Mus musculus
Samples (12)
Protocols (4)
Description
In cells derived from a genetically engineered Rb1 and Trp53 loss mouse model of SCLC (RP) expression of one of the three MYC family members induced from the endogenous locus using CRISPR activation. Cells were first stably transfected with the lenti-MS2-p65-HSF1 activator plasmid. Respective sgRNAs targeting either Myc, Mycl or MYCN were then cloned into the lentiSAMv2 system and transfected separately into the MS2-p65-HSF1 cells using lentiviral delivery.
Experiment types
RNA-seq of coding RNA, genotype design
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-7411.idf.txt
Sample and data relationshipE-MTAB-7411.sdrf.txt
Links