E-MTAB-7252 - Establishment of Porcine and Human Expanded Potential Stem Cells (ChIP-seq)

Status
Submitted on 16 September 2018, released on 1 April 2019, last updated on 16 April 2019
Organism
Homo sapiens, Sus scrofa
Samples (18)
Protocols (4)
Description
Despite intensive efforts, establishing porcine embryonic stem cells have been challenging. We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the activity of critical molecular pathways that predisposes lineage differentiation in the mouse preimplantation embryo. EPSCs had enriched molecular signatures of blastomeres and possessed the developmental potency to all embryonic and extraembryonic cell lineages. In this study, we report the derivation of porcine EPSC (pEPSC) lines either directly from preimplantation embryos or by reprogramming fetal fibroblasts. Under similar culture conditions, human ESCs and iPSCs can be converted, or somatic cells are directly reprogrammed, to EPSCs (hEPSCs) that display the molecular and functional attributes reminiscent of pEPSCs. Here, we performed ChIP-seq experiments of H3K4me3 and H3K27me3 to characterise the epigenetic signatures of the EPSCs.
Experiment types
ChIP-seq, binding site identification design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-7252.idf.txt
Sample and data relationshipE-MTAB-7252.sdrf.txt
Links