E-MTAB-7019 - Time series experiment on L4 stage of Caenorhabditis elegans N2

Status
Last updated on 24 July 2018, released on 24 July 2018
Organism
Caenorhabditis elegans
Samples (20)
Array (1)
Protocols (5)
Description
This experiment investigates the transcriptional effect of age in the N2 strain of Caenorhabditis elegans during the L4 stage. The goal is to identify genes that show dynamic gene-expression patterns over time. We grew N2 populations over the course of 44 to 58 hours at 20 degrees Celsius, independent populations were harvested in hourly intervals. Thereafter RNA was isolated, labelled and hybridized on microarray. The gene expression profiles were analyzed for dynamic expression patterns using principal component analysis and k-means clustering.
Experiment types
transcription profiling by array, time series design
Contact
Citation
A rapid and massive gene expression shift marking adolescent transition in C. elegans. Snoek LB, Sterken MG, Volkers RJ, Klatter M, Bosman KJ, Bevers RP, Riksen JA, Smant G, Cossins AR, Kammenga JE.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-7019.idf.txt
Sample and data relationshipE-MTAB-7019.sdrf.txt
Raw data (1)E-MTAB-7019.raw.1.zip
Array designA-GEOD-10094.adf.txt
Links