E-MTAB-7016 - Transcriptional and physiological diurnal responses of Tetradesmus obliquus under light-dark cycles and nitrogen limitation

Status
Last updated on 18 July 2018, released on 30 May 2019
Organism
Tetradesmus obliquus
Samples (41)
Protocols (5)
Description
This study is a follow up analysis of Tetradesmus obliquus 16:8 hours diurnal cycle experiments, where a starch-less mutant is compared to the wild-type, but this time, in nitrogen limited condition. The samples were collected approximately every 3 hours for both strains (wild-type and starchless mutant). Two time points per condition have two duplicate set (4 samples) because each condition was done in two batches (allowing to take samples during dark period). Overall, this study contain 41 samples, 20 for the wild-type and 21 for the starchless mutant. RNA was extracted for each samples and sent to Novogene for sequencing.
Experiment types
RNA-seq of coding RNA, growth condition design, strain or line design, time series design
Contacts
Citations
Draft Genome Sequence of the Oleaginous Green Alga Tetradesmus obliquus UTEX 393. B. M. Carreresa, L. de Jaegerb, J. Springerc, M. J. Barbosab, G. Breuerb, E. J. van den Endb, D. M. M. Kleinegrisc, I. Schäffersd, E. J. H. Wolbertc, H. Zhangd, P. P. Lamersb, R. B. Draaismad, V. A. P. Martins dos Santosa,e, R. H. Wijffelsb,f, G. Egginkb,c, P. J. Schaapa, D. E. Martensb. , PMID:28104651
The diurnal transcriptional landscape of the microalga Tetradesmus obliquus. Benoit M. Carreres, G. Mitsue León-Saiki, Peter J.Schaap, Vitor A.P. Martins dos Santos, René H. Wijffels, Dirk E. Martens, Maria Suarez-Diez.
The role of starch as transient energy buffer in synchronized microalgal growth in Acutodesmus obliquus. G. MitsueLeón-Saikia1Ilse M.Remmersa1Dirk E.MartensaPacko P.LamersaRené H.WijffelsabDouwevan der Veena.
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-7016.idf.txt
Sample and data relationshipE-MTAB-7016.sdrf.txt
Links