E-MTAB-7008 - Endoderm Differentiation - Single Cell RNA-sequencing
Last updated on 8 February 2019, released on 9 February 2019
Single-cell RNA-seq (scRNA-seq) on nocodazole and DMSO treated cells before and after differentiation into endoderm. hPSC colonies were treated with DMSO or 100ng/ml nocodazole for 16 hours and induced to differentiate into definitive endoderm for three days. Single cells were subsequently collected in either undifferentiated conditions (Und) or after 3 days of endoderm differentiation (Endoderm) and then sorted onto 384 well plates for Smart-Seq2 processing.
RNA-seq of coding RNA from single cells, development or differentiation design