E-MTAB-6984 - SCRB-seq comparison with TruSeq on bulk RNA-seq human LCL (DMSO) and BAY treated LCL (BAY)
Released on 7 July 2018, last updated on 24 April 2019
Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bulk RNA-seq procedure. For this we first gauge the applicability of the SCRB-seq protocol on bulk DMSO and BAY treated human LCL, and compare its effectiveness as compared to TruSeq.
RNA-seq of coding RNA, compound treatment design
BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. Alpern D, Gardeux V, Russeil J, Mangeat B, Meireles-Filho ACA, Breysse R, Hacker D, Deplancke B. 20(1):71 (2019)