E-MTAB-6946 - Single-cell RNA sequencing of mouse germ cells

Status
Released on 25 January 2019, last updated on 11 March 2019
Organism
Mus musculus
Samples (17)
Protocols (6)
Description
Spermatogenesis is a recurring differentiation process that results in the production of male gametes within the testes. During this process, spermatogonial stem cells differentiate to form spermatocytes, which undergo two rounds of meiotic division to form haploid spermatids. Throughout spermiogenesis, round spermatids elongate to form mature sperm. To profile maturing germ cells during the first wave of spermatogenesis, we generated droplet-based single cell RNAseq from juvenile animals at post-natal day P5-P35 as well as adult animals. Cells were isolated from whole testes. Furthermore, to assay the robustness of the meiotic cell division process, we profiled germ cells of the trans-chromosomal mouse model (Tc1) that carries a copy of the human chromosome 21.
Experiment types
RNA-seq of coding RNA from single cells, development or differentiation design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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