E-MTAB-6714 - A simple and robust plate-based single-cell ATAC-seq on various tissues and cell lines
Submitted on 25 January 2018, last updated on 9 October 2018, released on 10 October 2018
Homo sapiens, Mus musculus, mixed sample
The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, only a few studies have been done at the single cell level (scATAC-seq) due to technical difficulties. Here we developed a simple and robust plate-based scATAC-seq method, combining upfront bulk tagmentation with single-nuclei sorting, to investigate open chromatin regions. We applied this method on mouse splenocytes and unbiasedly revealed key regulatory regions and transcription factors that define each cell (sub)type.
ATAC-seq, binding site identification design