E-MTAB-6413 - Dual RNA-seq of peripheral blood from Gambian children with severe or uncomplicated Plasmodium falciparum malaria

Status
Released on 20 June 2018, last updated on 6 August 2019
Organism
Homo sapiens
Samples (46)
Protocols (4)
Description
This study examined the differences in human and parasite gene expression pattern between children with severe malaria and those with uncomplicated malaria through a dual RNA-seq approach. Peripheral blood samples were collected which contained substantial numbers of parasites that required no RNA enrichment prior to library preparation and sequencing.
Experiment types
RNA-seq of coding RNA, case control design, disease state design
Contact
Citations
Modelling pathogen load dynamics to elucidate mechanistic determinants of host–Plasmodium falciparum interactions. Athina Georgiadou, Hyun Jae Lee, Michael Walther, Anna E. van Beek, Fadlila Fitriani, Diana Wouters, Taco W. Kuijpers, Davis Nwakanma, Umberto D’Alessandro, Eleanor M. Riley, Thomas D. Otto, Azra Ghani, Michael Levin, Lachlan J. Coin, David J. Conway, Michael T. Bretscher & Aubrey J. Cunnington. , PMID:31209307
Integrated pathogen load and dual transcriptome analysis of systemic host-pathogen interactions in severe malaria. Hyun Jae Lee, Athina Georgiadou, Michael Walther, Davis Nwakanma, Lindsay B. Stewart, Michael Levin, Thomas D. Otto, David J. Conway, Lachlan J. Coin1, and Aubrey J. Cunnington. , PMID:29950443
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-6413.idf.txt
Sample and data relationshipE-MTAB-6413.sdrf.txt
Links