ArrayExpress

Last updated on 27 August 2018, released on 27 August 2018

Mus musculus

We have used ATAC-seq to track cell state changes that occur during the differentiation of mouse embryonic stem cells to defined neural progenitor fates. We have performed ATAC-seq every 24 hours in cells en route to 3 distinct neural progenitors fates, anterior, hindbrain and spinal cord. This has allowed us to define how cells transition to a neural state, based on their enhancer usage. We identified regions distinct to different anterior-posterior neural progenitors, and validated their relevance by performing in vivo ATAC-seq on neural progenitors isolated from different axial levels of mouse embryos.

ATAC-seq 

Sebastian Steinhauser <sebastian.steinhauser@crick.ac.uk>, Vicki Metzis <vicki.metzis@crick.ac.uk>

Regionalization of the nervous system requires axial allocation prior to neural lineage commitment. Vicki Metzis, Sebastian Steinhauser, Edvinas Pakanavicius, Mina Gouti, Despina Stamataki, Robin Lovell-Badge, Nicholas M Luscombe and James Briscoe.