E-MTAB-6337 - ATAC-seq on ESCs undergoing directed differentiation towards defined neural fates
Last updated on 27 August 2018, released on 27 August 2018
We have used ATAC-seq to track cell state changes that occur during the differentiation of mouse embryonic stem cells to defined neural progenitor fates. We have performed ATAC-seq every 24 hours in cells en route to 3 distinct neural progenitors fates, anterior, hindbrain and spinal cord. This has allowed us to define how cells transition to a neural state, based on their enhancer usage. We identified regions distinct to different anterior-posterior neural progenitors, and validated their relevance by performing in vivo ATAC-seq on neural progenitors isolated from different axial levels of mouse embryos.
Regionalization of the nervous system requires axial allocation prior to neural lineage commitment. Vicki Metzis, Sebastian Steinhauser, Edvinas Pakanavicius, Mina Gouti, Despina Stamataki, Robin Lovell-Badge, Nicholas M Luscombe and James Briscoe.