E-MTAB-6318 - Senataxin resolves R-loops forming at DNA double strand breaks to prevent translocations
Last updated on 20 February 2018, released on 20 February 2018
DNA Double Strands Breaks (DSBs) are highly detrimental since they can lead to mutations and chromosomes rearrangements (amplification, deletion, translocation and chromosome loss). Here, we set to assess the role of senataxin, a RNA:DNA helicase involved in the regulation of transcription and the maintenance of genome integrity, at sites of DNA Double Strand Breaks. We performed ChIP-Seq mapping of senataxin before and after damage, genome-wide DNA:RNA hybrids (DRIP) mapping before and after damage. We also performed RNA-Seq and RNA pol II mapping (total and phosphorylated on serine 2 of the CTD) by ChIP-Seq in undamaged cells to discriminate between damage in active versus inactive regions.
ChIP-seq, RNA-seq of coding RNA
Gaëlle Legube <firstname.lastname@example.org>, Marion Aguirrebengoa <email@example.com>, Nadine Puget <firstname.lastname@example.org>, Philippe Pasero, Sarah Cohen <email@example.com>, Thomas Clouaire <firstname.lastname@example.org>, Vincent Rocher <email@example.com>, Yea-Lih Lin, Yvan Canitrot
Senataxin resolves RNA:DNA hybrids forming at DNA double-strand breaks to prevent translocations. Cohen S, Puget N, Lin YL, Clouaire T, Aguirrebengoa M, Rocher V, Pasero P, Canitrot Y, Legube G. 9(1):533 (2018), PMID:29416069