E-MTAB-6318 - Senataxin resolves R-loops forming at DNA double strand breaks to prevent translocations

Status
Last updated on 20 February 2018, released on 20 February 2018
Organism
Homo sapiens
Samples (7)
Protocols (5)
Description
DNA Double Strands Breaks (DSBs) are highly detrimental since they can lead to mutations and chromosomes rearrangements (amplification, deletion, translocation and chromosome loss). Here, we set to assess the role of senataxin, a RNA:DNA helicase involved in the regulation of transcription and the maintenance of genome integrity, at sites of DNA Double Strand Breaks. We performed ChIP-Seq mapping of senataxin before and after damage, genome-wide DNA:RNA hybrids (DRIP) mapping before and after damage. We also performed RNA-Seq and RNA pol II mapping (total and phosphorylated on serine 2 of the CTD) by ChIP-Seq in undamaged cells to discriminate between damage in active versus inactive regions.
Experiment types
ChIP-seq, RNA-seq of coding RNA 
Contacts
Citation
Senataxin resolves RNA:DNA hybrids forming at DNA double-strand breaks to prevent translocations. Cohen S, Puget N, Lin YL, Clouaire T, Aguirrebengoa M, Rocher V, Pasero P, Canitrot Y, Legube G. 9(1):533 (2018), PMID:29416069
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-6318.idf.txt
Sample and data relationshipE-MTAB-6318.sdrf.txt
Links