E-MTAB-6116 - Single-cell ATAC-seq of a mixture of three oesophageal tissue (HET1A, OE33, OE19) derived cell lines
Submitted on 7 November 2016, last updated on 1 October 2018, released on 1 October 2018
Three oesophageal tissue derived cell lines, one from a normal tissue (HET1A) and two from tumour tissues (OE33 and OE199) were mixed with same number of each cell type in the same tube to get a mixed population. The C1 platform (Fluidigm) was used to capture single-cells and scATAC-seq protocols from Fluidigm ScriptHub is then used to generate the sequencing library. A single-cell ATAC-seq Bioinformatics pipeline is then developed to deconvolute the cells into their respective cell types.
ATAC-seq, cell type comparison design