E-MTAB-6051 - Single-cell RNA sequencing of OT-I CD8+ T cells after stimulation with different affinity ligands
Submitted on 7 February 2017, released on 25 June 2018, last updated on 28 January 2019
Activation of CD8+ T cells depends exquisitely on the affinity of the T cell receptor (TCR) for a peptide MHC (pMHC) ligand complex. Here, we activated OT-I transgenic CD8+ T cells with pure peptide and examined early activation responses by single-cell RNA-sequencing. T cells were activated with the high affinity OT-I cognate peptide (N4=SIINFEKL) for 1, 3 or 6 hours, or with reduced affinity peptides (T4=SIITFEKL and G4=SIIGFEKL) or the non-binding peptide (NP68=ASNENMDAM) for 6 hours. Cells were then sorted into 96-well plates by FACS and RNA was sequenced following an adapted Smart-Seq2 protocol.
RNA-seq of coding RNA, stimulus or stress design
T cell cytolytic capacity is independent of initial stimulation strength. Arianne C. Richard, Aaron T. L. Lun, Winnie W. Y. Lau, Berthold Göttgens, John C. Marioni & Gillian M. Griffiths. , PMID:30013148