E-MTAB-5779 - Gene expression profiling in control, heat-shock, and recovery treatment in a RIL population of Caenorhabditis elegans L4 larvae

Status
Released on 30 May 2017, last updated on 22 April 2021
Organism
Caenorhabditis elegans
Samples (168)
Array (1)
Protocols (5)
Description
This experiment investigates the genetic architecture of gene expression (eQTL) in three different treatments in a N2xCB4856 RIL population of Caenorhabditis elegans. The goal is to identify genetic variation linked to differences in gene expression. We exposed 48 RILs per treatment to a control, heat-stress, and recovery treatment. More specifically these three conditions can be characterized as: (i) the control treatment was grown for 48 hours at 20C, (ii) the heat-stress treatment was grown for 46 hours at 20C followed by 2 hours at 35C, and (iii) the recovery treatment was grown for 46 hours at 20C, followed by 2 hours at 35C and thereafter 2 hours at 20C. Thereafter RNA was isolated, labelled and hybridized on microarray. The gene expression profiles were used for eQTL mapping.
Experiment types
transcription profiling by array, stimulus or stress design, strain or line design
Contact
Citations
Contribution of trans regulatory eQTL to cryptic genetic variation in C. elegans. Snoek BL, Sterken MG, Bevers RPJ, Volkers RJM, Van't Hof A, Brenchley R, Riksen JAG, Cossins A, Kammenga JE. 18(1):500 (2017), PMID:28662696
Dissecting the eQTL Micro-Architecture in <i>Caenorhabditis elegans</i>. Sterken MG, Bevers RPJ, Volkers RJM, Riksen JAG, Kammenga JE, Snoek BL. 11:501376 (2020), PMID:33240309
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