E-MTAB-5655 - Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
Last updated on 1 December 2017, released on 2 December 2017
In this experiment the transcriptome reprogramming in barley during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old barley plants of cv. Vada were mock-inoculated or inoculated with P. hordei (Ph), 1.2.1 isolate, or P. triticina (Pt), BRW96258 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays.
transcription profiling by array, disease state design, time series design
A comparative analysis of nonhost resistance across the two Triticeae crop species wheat and barley. Rhoda Delventhal, Jeyaraman Rajaraman, Francesca L. Stefanato, Sajid Rehman, Reza Aghnoum, Graham R. D. McGrann, Marie Bolger, Björn Usadel, Pete E. Hedley, Lesley Boyd, Rients E. Niks, Patrick Schweizer and Ulrich Schaffrath. 17:232 (2017)