E-MTAB-5655 - Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease

Status
Last updated on 1 December 2017, released on 2 December 2017
Organism
Hordeum vulgare
Samples (36)
Array (1)
Protocols (5)
Description
In this experiment the transcriptome reprogramming in barley during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old barley plants of cv. Vada were mock-inoculated or inoculated with P. hordei (Ph), 1.2.1 isolate, or P. triticina (Pt), BRW96258 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays.
Experiment types
transcription profiling by array, disease state design, time series design
Contacts
Citation
A comparative analysis of nonhost resistance across the two Triticeae crop species wheat and barley. Rhoda Delventhal, Jeyaraman Rajaraman, Francesca L. Stefanato, Sajid Rehman, Reza Aghnoum, Graham R. D. McGrann, Marie Bolger, Björn Usadel, Pete E. Hedley, Lesley Boyd, Rients E. Niks, Patrick Schweizer and Ulrich Schaffrath. 17:232 (2017)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-5655.idf.txt
Sample and data relationshipE-MTAB-5655.sdrf.txt
Raw data (1)E-MTAB-5655.raw.1.zip
Array designA-MEXP-1728.adf.txt