E-MTAB-5640 - RNA sequencing of tumor associated macrophages and T cells in clear cell renal cell carcinoma
Submitted on 1 February 2017, released on 4 May 2017, last updated on 8 June 2017
Tumor associated macrophages and T cells were characterized in a cohort of 73 ccRCC and 5 healthy donors using mass cytometry. Macrophage and T cell subsets of interest were identified and sorted directly from samples known to contain a high frequency of these subsets. CD8+ PD-1- T cells (n=6), exhausted CD8+ PD-1+ T cells (n=6), control macrophages CD14+ HLA-DR+ CD204- CD38- CD206- (n=11) and a population of TAM characterized as CD14+ HLA-DR+ CD204+ CD38+ CD206- (n=6) were isolated using FACS sorting.
RNA-seq of coding RNA, cell type comparison design
An immune atlas of clear cell renal cell carcinoma. Stéphane Chevrier, Jacob H. Levine, Vito R.T. Zanotelli, Karina Silina, Daniel Schulz, Marina Bacac, Carola Ries, Laurie Ailles, Michael A.S. Jewett, Holger Moch, Maries van den Broek, Christian Beisel, Michael B. Stadler, Craig Gedye, Bernhard Reis, Dana Peer and Bernd Bodenmiller.