E-MTAB-5579 - Microarray experiment of murine macrophages co-cultured with neural stem cells in the presences of LPS
Submitted on 7 October 2016, last updated on 16 March 2017, released on 1 January 2018
This experiment aimed at characterising the modulatory role of murine induced Neural Stem Cells (iNSCs) and Neural Stem Cells (NSCs) on macrophages (MPs) exposed to LPS in vitro. Naïve MPs were polarized into an M1-like phenotype with LPS, and then co-cultured with 1:1 ratios of iNSCs in a trans-well system that avoids cell-to-cell contacts. Naïve MPs, LPS-stimulated MPs and LPS-stimulated MPs co-cultured with NSCs were used as controls.
transcription profiling by array, case control design, replicate design