E-MTAB-5285 - RNA-seq of A431 cell line after gefitinib treatment

Status
Submitted on 16 November 2011, last updated on 30 November 2016, released on 1 December 2016
Organism
Homo sapiens
Samples (12)
Protocols (6)
Description
RNA sequencing of A431 cell line samples before and after gefitinib treatment, at 0, 2, 6 and 24 hours, was performed in order to characterize the cell line's early and late response to this drug, and to compare against proteomics (mass spectrometry) characterization of the cell line using the same setup. These data were used in Branca et al., HiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics., Nat Methods. 2014 Jan;11(1):59-62 (doi: 10.1038/nmeth.2732).
Experiment types
RNA-seq of coding RNA, time series design
Contacts
Citation
HiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics. Branca RM, Orre LM, Johansson HJ, Granholm V, Huss M, Prez-Bercoff , Forshed J, Kll L, Lehti J. , PMID:24240322
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-5285.idf.txt
Sample and data relationshipE-MTAB-5285.sdrf.txt
Links