E-MTAB-5252 - RNA-Seq of Drosophlia adult mid-gut with the induction of RNAi against the largest subunit of RNA polymerase III

Status
Submitted on 1 August 2016, last updated on 15 November 2017, released on 29 November 2017
Organism
Drosophila melanogaster
Samples (6)
Protocols (5)
Description
TIGS>dC160RNAi females were fed RU486 from day 2 of adulthood to induce the RNAi expression, 10 guts were dissected per sample (the dissections were batched), RNA extracted with TRIZOL, cleaned up with Quiagen RNEasy with on-column DNase treatment, rRNA depleted (Ribo Zero Gold) and sequenced on the Illumina platform
Experiment types
RNA-seq of coding RNA, compound treatment design, replicate design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-5252.idf.txt
Sample and data relationshipE-MTAB-5252.sdrf.txt
Links