E-MTAB-5048 - Meiotic recombination initiation hotspots in Arabidopsis genes and transposons (H3K4m3 ChIP-seq)

Status
Last updated on 5 June 2017, released on 5 June 2017
Organism
Arabidopsis thaliana
Samples (9)
Protocols (5)
Description
To investigate the relationship between meiotic recombination initiation and H3K4m3 in Arabidopsis, we generated and sequenced H3K4m3 ChIP libraries from meiotic stage floral buds in wild type, arp6 and met1. To produce high-resolution of H3K4m3 mapping, we used micrococcal nuclease (MNase) to digest chromatins that were cross-linked by formaldehyde for ChIP. This experiment provides for H3K4m3 maps with the resolution of mononucleosomal DNA level (~150 bp).
Experiment type
ChIP-seq 
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-5048.idf.txt
Sample and data relationshipE-MTAB-5048.sdrf.txt
Links