E-MTAB-5042 - Meiotic recombination initiation hotspots in Arabidopsis genes and transposons (MNase-seq)
Status | Last updated on 5 June 2017, released on 5 June 2017 | ||||||||||
Organism | Arabidopsis thaliana | ||||||||||
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Description | To analyse nucleosome positioning and occupancy we performed micrococcal nuclease digestion of Arabidopsis wild type (Col-0) chromatin and gel purified the resulting ~150 bp mononucleosomal DNA band. This DNA was used to generate a library and paired-end sequencing performed (MNase-seq). To further examine influence of SWR1 chromatin remodelling complex and DNA methylation on nucleosome occupancy, we repeated MNase-seq in Aarabidopsis arp6 and met1 mutant. | ||||||||||
Experiment type | MNase-seq | ||||||||||
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