E-MTAB-5042 - Meiotic recombination initiation hotspots in Arabidopsis genes and transposons (MNase-seq)

Status
Last updated on 5 June 2017, released on 5 June 2017
Organism
Arabidopsis thaliana
Samples (3)
Protocols (4)
Description
To analyse nucleosome positioning and occupancy we performed micrococcal nuclease digestion of Arabidopsis wild type (Col-0) chromatin and gel purified the resulting ~150 bp mononucleosomal DNA band. This DNA was used to generate a library and paired-end sequencing performed (MNase-seq). To further examine influence of SWR1 chromatin remodelling complex and DNA methylation on nucleosome occupancy, we repeated MNase-seq in Aarabidopsis arp6 and met1 mutant.
Experiment type
MNase-seq 
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-5042.idf.txt
Sample and data relationshipE-MTAB-5042.sdrf.txt
Links