E-MTAB-4918 - In situ Hi-C study of chromatin conformation during early Drosophila development

Status
Submitted on 31 October 2015, released on 6 April 2017, last updated on 14 January 2019
Organism
Drosophila melanogaster
Samples (45)
Protocols (10)
Description
In this experiment we examined higher order chromatin structure during early Drosophila melanogaster development. We performed in situ Hi-C for hand-sorted non-mitotic embryos at nuclear cycle number 12, 13 and 14, and for embryos at 3-4 hours post fertilisation. During this time in development, the zygotic genome is activated and zygotic transcription is taking place for the first time. To assess the impact of transcription on chromatin structure we injected the transcription inhibitors alpha-amanitin or triptolide before zygotic genome activation and performed Hi-C and ChIP-seq for RNA Pol II. Furthermore, we used Hi-C to study genome architecture in embryos lacking the transcription factor Zelda.
Experiment types
Hi-C, ChIP-seq, cell cycle design, compound treatment design, development or differentiation design, genotype design
Contacts
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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