E-MTAB-4888 - RNA-seq of coding RNA from single cells to assess the impact of aging on cell-to-cell transcriptional variability during immune stimulation
Submitted on 16 June 2016, released on 21 July 2016, last updated on 18 September 2018
We explored how aging impacts transcriptional dynamics using single-cell RNA-sequencing to profile hundreds of CD4+ T cells from young and old mice from two divergent species. In young animals, immunological challenge drives a conserved transcriptomic switch from highly variable to tightly regulated gene expression, characterized by a strong up-regulation of a core activation program, coupled with a decrease in cell-to-cell variability. Aging significantly perturbed the activation of this core program, and increased expression heterogeneity across the population of cells in both species.
RNA-seq of coding RNA from single cells, cell type comparison design, species comparison design
Aging increases cell-to-cell transcriptional variability upon immune stimulation. Celia Pilar Martinez-Jimenez1,2,*, Nils Eling1,3,*, Hung-Chang Chen1, Catalina A. Vallejos3,4, Aleksandra A. Kolodziejczyk2,3, Frances Connor1, Lovorka Stojic1, Timothy F. Rayner1, Michael J. T. Stubbington3, Sarah A. Teichmann2,3, Maike de la Roche1, John C. Marioni1,2,3,†, Duncan T. Odom1,2,†. , PMID:28360329