E-MTAB-483 - Transcription profiling by array of human ovarian granulosa cell tumor and normal granulosa cells

Status
Released on 2 August 2012, last updated on 3 June 2014
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (7)
Description
RNA was extracted from normal human granulosa cells from IVF patients (hGC1 and hGC2 samples) and from adult-type ovarian granulosa cell tumor samples (H1, H8, H20, H23, H24, H28, H30, H33, H4, H18) as described in Jamieson et al, 2010. RNA from all samples was linearly amplified using the Whole Transcriptome Amplification kit (Sigma), starting from 300ng of RNA, and with 12 amplifications cycles. cDNA was purified on columns and sent to the Nimblegen platform for hybridization and transcriptional profiling. The FOXL2 locus was gentoyped in tumor samples, and all samples were found positive for the recurrent somatic mutation p.Cys134Trp which is present in >95% of adult-type ovarian granulosa cell tumors (Shah et al, 2009).
Experiment types
transcription profiling by array, co-expression, disease state, in vivo
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-483.idf.txt
Sample and data relationshipE-MTAB-483.sdrf.txt
Raw data (1)E-MTAB-483.raw.1.zip
Processed data (2)E-MTAB-483.processed.1.zip, E-MTAB-483.processed.2.zip
Array designA-GEOD-10191.adf.txt