E-MTAB-483 - Transcription profiling by array of human ovarian granulosa cell tumor and normal granulosa cells
Released on 2 August 2012, last updated on 3 June 2014
RNA was extracted from normal human granulosa cells from IVF patients (hGC1 and hGC2 samples) and from adult-type ovarian granulosa cell tumor samples (H1, H8, H20, H23, H24, H28, H30, H33, H4, H18) as described in Jamieson et al, 2010. RNA from all samples was linearly amplified using the Whole Transcriptome Amplification kit (Sigma), starting from 300ng of RNA, and with 12 amplifications cycles. cDNA was purified on columns and sent to the Nimblegen platform for hybridization and transcriptional profiling. The FOXL2 locus was gentoyped in tumor samples, and all samples were found positive for the recurrent somatic mutation p.Cys134Trp which is present in >95% of adult-type ovarian granulosa cell tumors (Shah et al, 2009).
transcription profiling by array, co-expression, disease state, in vivo
Adult ovarian granulosa cell tumor transcriptomics: prevalence of FOXL2 target genes misregulation gives insights into the pathogenic mechanism of the p.Cys134Trp somatic mutation. Benayoun BA, Anttonen M, L'hote D, Bailly-Bechet M, Andersson N, Heikinheimo M, Veitia RA. Oncogene (2012), PMID:22797072