E-MTAB-4617 - Single-cell RNA sequencing of spleen-derived LCK-expressing cells from adult zebrafish

Status
Submitted on 13 June 2015, released on 1 November 2016, last updated on 19 July 2017
Organism
Danio rerio
Samples (864)
Protocols (4)
Description
Transcriptome data from individual lck:GFP expressing cells isolated from spleens of two adult Zebrafish. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T-cells and NK-like and a minor one of myeloid-like cells. Single cell transcriptomes are matched with FACS index sorting data (GFP, forward and side light scatter and dead cell staining)
Experiment type
RNA-seq of coding RNA from single cells 
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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