E-MTAB-4366 - Transcription profiling of T cells from healthy controls and patients with seasonal allergic rhinitis before and after one year of sublingual immunotherapy

Status
Last updated on 4 October 2016, released on 31 March 2017
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (7)
Description
We recruit 4 healthy controls (HC) and 4 patients (P) with seasonal allergic rhinitis before (b) sublingual immunotherapy at the same time and also the same patients after one year (a) of sublingual immunotherapy. Peripheral blood mononuclear cells (PBMCs) obtained from patients and controls were challenged with diluent (D) or allergen (A) extracts from birch pollen at a density of 106 cells/mL for 7 days in RPMI 1640 supplemented with 2 mM L-glutamine, 5% human AB serum, 5 μM β– mercaptoethanol and 50 μg/mL gentamicin. CD4+ T cells were isolated using flow cytometry and the quantity and quality of RNA was examined as described before. Gene expression microarrays (Illumina, San Diego, CA, USA) were performed (by using Agilent G4851B SurePrint G3 Hmn 8×60K V2 Microarray Kit).
Experiment type
transcription profiling by array 
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-4366.idf.txt
Sample and data relationshipE-MTAB-4366.sdrf.txt
Raw data (1)E-MTAB-4366.raw.1.zip
Array designA-MEXP-2320.adf.txt