E-MTAB-4304 - RNA-Seq analysis of human intact and damaged osteoarthrtitic cartilage following total knee replacement
Released on 6 April 2016, last updated on 4 December 2017
This experiment captures the expression of genes between two sites of human cartilage within the same patients to allow investigation of genomic responses to damage during osteoarthritis. Eight patients with symptomatic OA undergoing total knee replacement (n=8, age range 65-79 years, mean age 70.3) were used in this study. Cartilage from paired osteochondral samples were isolated from the intact PLC (posterior lateral condyle) and the damaged DMC (distal medial condyle) for RNA-seq analysis.
RNA-seq of coding RNA
Gene expression changes in damaged osteoarthritic cartilage identify a signature of non-chondrogenic and mechanical responses. Dunn SL, Soul J, Anand S, Schwartz JM, Boot-Handford RP, Hardingham TE. 24(8):1431-1440 (2016), PMID:26973327