E-MTAB-3861 - Evolutionary history of host, rather than plant phylogeny, determines gene expression in a generalist butterfly
Submitted on 18 August 2015, last updated on 9 September 2015, released on 18 November 2015
Here we studied Vanessa cardui, the species with the widest diet breadth among butterflies and a potential insect pest, by comparing tissue-specific transcriptomes from caterpillars that were fed six different host plants. We tested whether the similarities of gene-expression response reflect the evolutionary history of adaptation to these plants in the Vanessa and related genera, against the null hypothesis of transcriptional profiles reflecting plant phylogenetic relatedness. Science for Life Laboratory (SciLifeLab, Sweden) conducted the sequencing of RNA samples. The cDNA libraries (Illumina TruSeq RNA) were sequenced using the Illumina HiSeq 2000 platform using 100-bp paired-end sequencing. We obtained more than 9 million read-pairs from seventy one cDNA libraries sequenced and the transcriptome assembly (TA) of these sequences resulted in 213, 237 transcripts (162,189 components) with a contig N50 of 2,193 bp. Thus, we covered approximately 300x the transcriptome of caterpillars of the species V. cardui.
RNA-seq of coding RNA, organism part comparison design, replicate design, stimulus or stress design
Evolutionary history of host use, rather than plant phylogeny, determines gene expression in a generalist butterfly. Celorio-Mancera Mde L, Wheat CW, Huss M, Vezzi F, Neethiraj R, Reimegård J, Nylin S, Janz N. :59 (2016), PMID:26956800