E-MTAB-3470 - CD8 in vitro CD2-costimulation
Submitted on 1 April 2015, last updated on 12 August 2015, released on 12 August 2015
Purified primary human CD8 T cells were stimulated in vitro in parallel using a magnetic bead conjugated to either anti-CD3/28 or anti-CD2/3/28 antibodies. Cells were cultured for 6 days in complete RPMI1640 and 10ng/ml IL2 and were harvested on day 6 and RNA was isolated for further labelling and hybridisation.
transcription profiling by array, stimulus or stress design
T cell exhaustion, costimulation and clinical outcome in autoimmunity and infection. Eoin F. McKinney, James C. Lee, David R.W. Jayne, Paul A. Lyons, Kenneth G.C. Smith.