E-MTAB-3237 - Transcription profiling by array of pig peripheral blood mononuclear cells (PBMCs) vaccinated against porcine reproductive and respiratory syndrome (PRRS) to identify molecular pathways associated with "good" and "bad" PRRSV vaccine responders
Submitted on 16 August 2013, last updated on 27 November 2016, released on 28 November 2016
The initial interaction of porcine reproductive and respiratory virus (PRRSV) with the immune system is of critical importance for immunological and clinical outcomes. PRRSV infection is associated with inefficient or aberrant immune responses: weak and delayed neutralizing antibody responses and erratic IFN-γ producing T-cells specific response. The lack of vaccine correlates capable of predicting protection has largely hampered the development of new efficacious PRRS vaccines. The objective of this study was to determine if systems biology (1) could be used to identify molecular pathways associated with “good” and “bad” PRRSV vaccine responders in terms of their intensity of IFN- γ secreting cell responses.
transcription profiling by array, case control design, disease state design, reference design, replicate design