E-MTAB-2950 - RNA-seq of mouse 1-cell embryos

Status
Submitted on 20 September 2014, last updated on 19 March 2015, released on 19 March 2015
Organism
Mus musculus
Samples (8)
Protocols (5)
Description
Initiation of zygotic transcription in mammals is poorly understood. In mice, zygotic transcription is first detected shortly after pronucleus formation in 1-cell embryos but the identity of the transcribed loci and mechanisms regulating their expression are not known. Using total RNA-seq we find that transcription in 1-cell embryos is highly promiscuous such that intergenic regions are extensively expressed and thousands of genes are transcribed at relatively low levels.
Experiment types
RNA-seq of coding RNA, RNA-seq of non coding RNA, compound treatment design, time series design
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-2950.idf.txt
Sample and data relationshipE-MTAB-2950.sdrf.txt
Links