E-MTAB-2805 - Gene expression pattern of single mES cells during cell cycle stages
Released on 29 December 2014, last updated on 13 February 2015
In this study, we aimed to study the gene expression patterns at single cell level across the different cell cycle stages in mESC. We performed single cell RNA-Seq experiment on mESC that were stained with Hoechst 33342 and Flow cytometry sorted for G1, S and G2M stages of cell cycle. Single cell RNA-Seq was performed using Fluidigm C1 system and libraries were generated using Nextera XT (Illumina) kit.
RNA-seq of coding RNA from single cells, cell type comparison design, co-expression, in vitro
Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells. Florian Buettner, Kedar N Natarajan, F Paolo Casale, Valentina Proserpio, Antonio Scialdone, Fabian J Theis, Sarah A Teichmann, John C Marioni & Oliver Stegle. http://www.nature.com/nbt/journal/v33/n2/full/nbt.3102.html Volume 33 No 2:155 - 160 (2015), PMID:25599176