E-MTAB-2512 - Th single cell RNA-Seq

Released on 25 April 2014, last updated on 22 August 2016
Mus musculus
Samples (96)
Protocols (4)
Th2 cells regulate helminth infections, allergic disorders, tumor immunity and pregnancy by secreting various cytokines. It is likely that there are undiscovered Th2-signaling molecules. While steroids are known to be immunoregulators, de novo steroid production from immune cells has not been previously characterised. We demonstrate production of the steroid pregnenolone by Th2 cells in vitro, and in vivo in a helminth infection model. To identify gene-expression identify of these steroidogenic-Th2 cells, we performed single-cell RNA-sequencing.
Experiment types
RNA-seq of coding RNA from single cells, cell type comparison design, co-expression, in vitro
Single Cell RNA-Sequencing Reveals T helper Cells Synthesizing Steroids de novo to Contribute to Immune Homeostasis. Bidesh Mahata, Xiuwei Zhang, Aleksandra A. Kolodziejczyk, Valentina Proserpio, Liora Haim-Vilmovsky,Angela E. Taylor, Daniel Hebenstreit, Felix A. Dingler, Victoria Moignard, Berthold Göttgens, Wiebke Arlt, Andrew N J McKenzie and Sarah A. Teichmann. Cell Rep 7(4):1130-1142 (2014), PMID:24813893
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-MTAB-2512.idf.txt
Sample and data relationshipE-MTAB-2512.sdrf.txt
Additional data (1)E-MTAB-2512.additional.1.zip